Efficient enzymatic synthesis of the sialyl‐LewisX tetrasaccharide
- 20 September 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 330 (3) , 243-248
- https://doi.org/10.1016/0014-5793(93)80881-t
Abstract
Sialyl‐Lewisx (NeuAcα2→3Galβ1→4[Fucαl→3]GlcNAc] has been identified as a ligand for E‐selectin, P‐selectin and recently also for L‐selectin. We have synthesized the sialyl‐Lewisx tetrasaccharide by total enzymatic synthesis from N‐acetyllactosamine using a placental α2→3‐sialyltransferase specific for type‐2 chain acceptors, followed by a cloned human α1→3‐fucosyltransferase (FucTV, the ‘plasma‐type’ enzyme). This procedure resulted in the tetrasaccharide in a 61% overall yield.Keywords
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