Characterization of Virus-Specific RNAs from Subacute Sclerosing Panencephalitis Virus-Infected CV-1 Cells

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Abstract
CV-1 cells infected with subacute sclerosing panencephalitis (SSPE) virus incorporated uridine- 3 H into at least four virus-specific RNA components in the presence of actinomycin D. The component sedimenting fastest had a sedimentation coefficient of 50 s corresponding to a molecular weight of 6 × 10 6 . The other three RNA components have sedimentation constants of 35 s , 22 s , and 18 s corresponding to molecular weights of 2.5 × 10 6 , 1.0 × 10 6 , and 0.75 × 10 6 , respectively. The base composition of the 50 s RNA is distinct from that of cellular RNA and comparable with base compositions of viral RNAs of other paramyxoviruses. The base composition of the 18 s RNA shows approximate complementarity with the 50 s RNA. RNA-RNA annealing experiments using unlabeled 50 s SSPE RNA with labeled 18 s RNA from cells infected with SSPE virus or measles virus show 100% annealing with 18 s SSPE RNA but only 60% annealing with 18 s measles RNA. These experiments suggest some differences between the 18 s RNAs of SSPE virus-infected cells and measles virus-infected cells.

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