High efficiency of heterologous transformation of a restrictionless and modificationless mutant of Bacillus subtilis 168.

1 January 1980

journal article
Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry

Vol. 44 (3) , 621-623
https://doi.org/10.1271/bbb1961.44.621

Abstract

Efficiencies of transformation with heterologous DNAs were examined on B. subtilis 168-YSI1 and its restrictionless and modificationless mutant B. subtilis RM125. Whereas the efficiencies of homologous transformation on YS11 and RM125 were almost the same, RM125 showed 30-200 fold higher efficiencies than YS11 when heterologous DNAs were used as transforming DNAs.

Keywords

BACILLUS SUBTILIS
HETEROLOGOUS
FOLD HIGHER
HOMOLOGOUS TRANSFORMATION
RESTRICTIONLESS
TRANSFORMING DNAS
MODIFICATIONLESS
HIGHER EFFICIENCIES
EFFICIENCIES THAN
THAN YS11

This publication has 0 references indexed in Scilit: