SIMPLE AND RAPID RADIOIMMUNOASSAY OF TRIIODOTHYRONINE IN UNEXTRACTED SERUM

Google Scholar
  • 1 January 1976
    • journal article
    • research article
    • Vol. 17  (5) , 411-416
Abstract
A simple, accurate and rapid procedure for radioimmunoassay (RIA) of triiodothyronine (T3) is described. Serum is denatured by sodium trichloroacetate and the T4 released is allowed to react with 125I-T3-labeled T3 antiserum. The displaced 125I-T3 is rapidly taken up by an anionic-resin sponge, and this uptake is linearly related to serum T3 concentration. The entire assay procedure was completed in the same tube in about 1 h. The T3 antibody was specific, and thyroxine (T4)-to T3 conversion in the assay did not occur. Recovery of exogenously added T3 was virtually complete. The mean T3 concentration in 92 euthyroid subjects (142 ng/ 100 ml) is comparable to the values descried in a number of RIA for T3.

This publication has 1 reference indexed in Scilit: