Preparation and Use of a Quality Control Hemolysate for Microchromatographic Determinations of Hemoglobin A2
Open Access
- 1 March 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 67 (3) , 215-218
- https://doi.org/10.1093/ajcp/67.3.215
Abstract
Schmidt, Robert M., Brosious, Effie M., and Wright, Jane M.: Preparation and use of a quality control hemolysate for microchromatographic determinations of hemoglobin A2. Am J Clin Pathol 67:215–218, 1977. Compared with other methods for determining hemoglobin A2 (Hb A2) levels, the new microchromatography procedures are fast, easy laboratory technics useful for quantitating Hb A2 that can be readily monitored for quality control of results. A reliable method for preparing a stable hemolysate control for monitoring the accuracy and precision of Hb A2 microchromatographic procedures is described. The recombination of purified hemoglobin fractions in known concentrations and the addition of potassium cyanide and carbon monoxide produce a control solution that gives stable hemoglobin A2 values for at least 40 weeks. Variability among six reference laboratories was small.Keywords
This publication has 2 references indexed in Scilit:
- EFFECT OF IRON DEFICIENCY ON LEVELS OF HEMOGLOBINS A2 AND E1968
- Rapid electrophoresis and quantitation of haemoglobins on cellulose acetateJournal of Clinical Pathology, 1965