A Method for Measuring Plasma Levels of Digitalis Glycosides
- 1 February 1965
- journal article
- research article
- Published by Wolters Kluwer Health in Circulation
- Vol. 31 (2) , 228-233
- https://doi.org/10.1161/01.cir.31.2.228
Abstract
A method is described for measuring plasma levels of cardiac glycosides, utilizing their inhibitory effect on the rubidium-86 uptake of human red cells. As little as 0.05 mµg. of digoxin/ml. of plasma produces detectable inhibition, but due to the variable effect of other substances in human plasma the practical limit of the method is 1.0 mµg./ml., about 20 times as sensitive as the duck embryo heart or the C14-labeled technics. Patients on digitalis leaf were found to have much higher circulating levels than patients on digoxin (average value 7.5 mµg./ ml. of digoxin equivalent for digitalis leaf, 0.05 mµg./ml. for digoxin). Patients toxic on digoxin averaged 6 mµg./ml. Of patients not taking digitalis, 7 per cent had significant levels of digitalis-like substances in their plasma; these were mostly individuals with metastatic carcinoma, severe fluid and electrolyte disturbances, or heart failure. No drug (other than cardiac glycosides) gave consistent high levels by this test.Keywords
This publication has 6 references indexed in Scilit:
- Red Cell Preservation: Further Studies with AdenineBlood, 1962
- Membrane Adenosine Triphosphatase as a Participant in the Active Transport of Sodium and Potassium in the Human ErythrocyteJournal of Biological Chemistry, 1960
- The action of cardiac glycosides on sodium and potassium movements in human red cellsThe Journal of Physiology, 1957
- Mechanism of Staircase and Contracture in Ventricular MuscleAmerican Journal of Physiology-Legacy Content, 1953
- Employment of the Embryonic Duck Heart for the Detection of Minute Amounts of a Digitalis Glycoside (Lanatoside G)Experimental Biology and Medicine, 1947