Multiple, Tandem Plasmid Integration inSaccharomyces cerevisiae
Open Access
- 1 April 1983
- journal article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 3 (4) , 747-749
- https://doi.org/10.1128/mcb.3.4.747
Abstract
Nonreplicating plasmids transformSaccharomyces cerevisiaeby recombining with a homologous site in the genome. Frequently, multiple copies of the plasmid integrate in a tandem array. We show that, after transformation with restriction enzyme-cut plasmids, most, if not all, multimers arise by sequential integration of plasmid molecules into the same genomic location.Keywords
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