Reversible inactivation of endothelial nitric oxide synthase by NG‐nitro‐l‐arginine
- 25 October 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 333 (1-2) , 203-206
- https://doi.org/10.1016/0014-5793(93)80405-j
Abstract
N G-Methyl-l-arginine (L-NMA) and N G-nitro-l-arginine (L-NNA) inhibited NO-induced cGMP accumulation in porcine aortic endothelial cells with half-maximally effective concentrations of 15 and 3.4 μM, respectively. The effects of both compounds were reversible, but the L-NNA-induced inhibition was only reversed by wash-out in the presence of 1 mM l-arginine. In short-term incubations (45 s) of membrane fractions, L-NMA and L-NNA exhibited similar potencies to inhibit endothelial NO synthase, but L-NNA was markedly more potent than L-NMA after prolonged incubation periods (⩾ 3 min) due to induction of a pronounced, reversible enzyme inactivation.Keywords
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