Function of saposin C in the reconstitution of glucosylceramidase by phosphatidylserine liposomes
- 20 December 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 336 (1) , 159-162
- https://doi.org/10.1016/0014-5793(93)81631-9
Abstract
The function of saposin C (Sap C), a glucosylceramidase activator protein, in the enzyme stimulation by phosphatidylserine (PS) liposomes has been investigated. Using gel filtration experiments evidence was obtained for Sap C binding to PS large unilamellar vesicles (LUV) but not to glucosylceramidase. PS LUV, which by themselves are unable to tightly bind and stimulate the enzyme, acquire the capacity to also bind the enzyme after interaction with Sap C, making it express its full activity. Our results indicate that the primary step in the Sap C mode of action resides in its association with PS membranes; in turn, this association promotes the interaction between the membranes and glucosylceramidase.Keywords
This publication has 22 references indexed in Scilit:
- Studies on glucosylceramidase binding to phosphatidylserine liposomes: the role of bilayer curvatureBiochimica et Biophysica Acta (BBA) - Biomembranes, 1993
- Activator proteins and topology of lysosomal sphingolipid catabolismBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1992
- Reconstitution of glucosylceramidase on binding to acidic phospholipid-containing vesiclesBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1992
- Mutation in the sphingolipid activator protein 2 in a patient with a variant of Gaucher diseaseFEBS Letters, 1991
- Conditions affecting the activity of glucocerebrosidase purified fom spleens of control subjects and patients with type 1 Gaucher diseaseBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1990
- Surfactant protein SP-B induces ordering at the surface of model membrane bilayersBiochemistry, 1990
- Correlation between the activity of glucosylceramidase and its binding to glucosylceramide-containing liposomes. Role of acidic phospholipids and fatty acidsBiochimica et Biophysica Acta (BBA) - General Subjects, 1990
- The Precursor of Sulfatide Activator Protein is Processed to Three Different ProteinsBiological Chemistry Hoppe-Seyler, 1988
- Mechanism of activation of glucocerebrosidase by CO-β-glucosidase (glucosidase activator protein)Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1981
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970