Spectrofluorometric Analysis of Cardiotonic Steroids
- 1 September 1961
- journal article
- Published by Oxford University Press (OUP) in Journal of Pharmacy and Pharmacology
- Vol. 13 (1) , 389-395
- https://doi.org/10.1111/j.2042-7158.1961.tb11843.x
Abstract
The sensitive and specific fluorimetric methods introduced by Jensen (1952; 1953) for digitalis estimation were re-evaluated for the assay of cardiotonic steroids and other steroids of biological interest. Characteristics of the excitation and fluorescence spectra were determined. Using a modification of Jensen's original digitoxin method and a spectrofluorometer, a linear relation was demonstrated between fluorescence intensity and concentrations from 0.01 to 6 μg./ml. for both digitoxin and digoxin. Application of these methods to the assay of gitoxin and cholesterol would seem to be equally practicable. In addition, scillaren A and B and strophanthin K can be made to fluoresce weakly. On the other hand, ouabain, cholestanol, testosterone, and hydrocortisone showed no fluorescence. Possible mechanisms of the reactions are discussedKeywords
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