Quadroma-Secreted Bi(Interferon Alpha 2—Peroxidase) Specific Antibody Suitable for One-Step Immunoassay

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Abstract
A mouse hybridoma (quadroma) was prepared by fusing hybridomas producing monoclonal antibody of G1-isotype to human interferon-alpha2 with hybridomas producing monoclonal antibody of G2a-isotype against horseradish peroxidase. The established quadroma line secreted immunoglobulins of both Gl/G2a-isotypes which manifested parental and bispecific binding characteristics. Culture supernatant containing the bifunctional antibody cross-linking interferon and peroxidase was used for a one-step immunoassay. The developed sandwich ELISA was able to detect the human interferon-alpha2 at a concentration of 10 units/ml (0,1 ng/ml) within 2-3 hours.