Observations on Preservation of Human Spermatozoa at Low Temperatures
- 1 April 1953
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 82 (4) , 686-688
- https://doi.org/10.3181/00379727-82-20219
Abstract
When 9 parts of liquefied human semen are mixed with 1 of absolute glycerol and placed in an insulated box containing dry ice, it can be stored for at least 3 months, After quick thawing in a 37[degree]C water bath, there is an avg. spermatozoal survival of 67 %, a figure superior to those realized with faster freezing methods. Such revived spermatozoa show no detectable alterations in motility or morphology.Keywords
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