Proliferation of human embryonic corneal stromal cells in a serum-free medium
- 30 June 1985
- journal article
- research article
- Published by Wiley in Cell Biology International Reports
- Vol. 9 (7) , 655-662
- https://doi.org/10.1016/0309-1651(85)90058-x
Abstract
Substantial multiplication of stromal cells from human embryonic corneas has been obtained in a basal medium MCDB 104 supplemented with 25 ng EGF/ml, 10 .mu.g insulin/ml, 20 .mu.g transferrin/ml, 25 ng MSA/ml, 500 .mu.g ovalbumin/ml, 50 .mu.g LDL/ml, 50 .mu.g HDL/ml and 10-6 M hydrocortisone. Eventhough the growth rate appears to be similar to that in 10% serum, the cells cease proliferating at a lower density.This publication has 8 references indexed in Scilit:
- Primary cell cultures from human embryonic corneasJournal of Cell Science, 1984
- Serum‐free culture of PC13 murine embryonal carcinoma cellsJournal of Cellular Physiology, 1983
- Growth of human foreskin fibroblasts in a serum‐free, defined medium without platelet‐derived growth factorJournal of Cellular Physiology, 1982
- Rapid clonal growth and serial passage of human diploid fibroblasts in a lipid-enriched synthetic medium supplemented with epidermal growth factor, insulin, and dexamethasone.Proceedings of the National Academy of Sciences, 1981
- Growth regulation of WI38 cells in a serum-free mediumExperimental Cell Research, 1981
- Mass Culture of Human Corneal Endothelial CellsArchives of Ophthalmology (1950), 1979
- Purification of human platelet-derived growth factor.Proceedings of the National Academy of Sciences, 1979
- Purification of the fibroblast growth factor activity from bovine brain.Journal of Biological Chemistry, 1978