Inhibition of protein synthesis by the cordycepin analog of (2′–5′)ppp(Ap)nA, (2′–5′)ppp(3′dAp)n3′ dA, in intact mammalian cells

Abstract
The introduction of the cordycepin analog of (2′–5′)An, (2′–5′)ppp(3′ dAp)n3′ dA [referred to as (2′–5′)p33′ dAn], into mouse L929 cells and cultured human fibroblasts resulted in a dose-dependent inhibition of protein synthesis which was comparable to the inhibition observed by (2′–5′)ppp(Ap)nA [referred to as (2′–5′)p3An]. The inhibition of protein synthesis by (2′–5′)p33′ dAn was much more persistent than that of the naturally occurring (2′–5′)p3An following prolonged incubation of cells. Furthermore, the (2′–5′)p3An was cytotoxic to mammalian cells in culture, whereas the (2′–5′)p33′dAn was not

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