Production of L-Malic Acid by Permeabilized Cells of Commercial Saccharomyces Sp. Strains
- 1 December 2005
- journal article
- Published by Springer Nature in Biotechnology Letters
- Vol. 27 (23-24) , 1835-1839
- https://doi.org/10.1007/s10529-005-3890-3
Abstract
Of various yeasts tested in the conversion of fumaric to l-malic acid, Saccharomyces bayanus had the highest activity of fumarase. Cells permeabilized with 0.2% (w/v) CTAB for 5 min gave maximum enzyme activity. Under non-growth conditions, fumarase activity in the permeabilized cells was four times higher (271 U/g) than that of the intact cells (67 U/g). The proposed mathematical model for the batch production of l-malic acid was validated at different initial fumaric acid concentrations. The average conversion of fumaric acid was up to 82% and gave 21, 40, 83 and 175 mm l-malic acid from respectively, 25, 50, 100 and 210 mm fumaric acid.Keywords
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