Transcriptional Regulation of Rat Insulin-Like Growth Factor-Binding Protein-1 Expression by Growth Hormone
Open Access
- 1 August 1990
- journal article
- research article
- Published by The Endocrine Society in Molecular Endocrinology
- Vol. 4 (8) , 1199-1204
- https://doi.org/10.1210/mend-4-8-1199
Abstract
The low mol wt insulin-like growth factor-binding protein (IGFBP-1) originally isolated from amniotic fluid has been considered to be GH independent. In this report we have examined the effect of GH on hepatic IGFBP-1 expression in the hypophysectomized rat. Using a rat IGFBP-1 cDNA probe we now demonstrated that hepatic IGFBP-1 expression is up-regulated in the GH-deficient rat. In addition, using ligand blotting, an increase in the abundance of a 30-kDa [125I]IGF-I-BP was detected in both hepatic extracts and serum from hypophysectomized rats. After a single ip injection of GH, the IGFBP-1 transcription rate was reduced within 30 min to the levels seen in the sham-operated control rats. Similarly, hepatic IGFBP-1 mRNA abundance was reduced after both acute GH injection and chronic GH treatment for 8 days. These data demonstrate that IGFBP-1 expression is inversely regulated by GH.Keywords
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