Carbonic anhydrase as a tool in studying the mechanism of reactions involving H2CO3, CO2 or HCO3−

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Abstract
[long dash]When a powerful Jack bean urease prepn. was allowed to act upon a limited quantity of urea in a neutral buffer at 15[degree]C, CO3 was rapidly evolved and later gradually reabsorbed. If ox blood carbonic anhydrase was added, no excess of CO2 was evolved. These results support the conception that CO2, and not bicarbonate or carbonate is a primary intermediate in the action of urease on urea. Carbonic anhydrase had a similar effect in the yeast carboxylase-pyruvate system under certain described conditions. The results indicated that CO2, not bicarbonate or carbonic acid is the primary product in the enzymic decarboxylation of pyruvate. The effects of adding carbonic anhydrase to systems in which CO2 or bicarbonate are reactants and the use of Warburg manometers for detn. of carbonic anhydrase activity were discussed.

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