A Monoclonal Antibody-Based Immunoradiometric Assay for Low Molecular Weight Insulin-Like Growth Factor Binding Protein/Placental Protein 12
- 1 December 1989
- journal article
- research article
- Published by Taylor & Francis in Journal of Immunoassay
- Vol. 10 (4) , 325-337
- https://doi.org/10.1080/01971528908053245
Abstract
We describe a sensitive immunoradiometric assay for insulin-like growth factor binding protein/Placental Protein 12 (IGF-BP/PP12) using monoclonal antibodies. This assay has a detection limit of 0.25 μg/l IGF-BP/PP12. Parallel dose response curves were obtained with purified IGF-BP/PP12, amniotic fluid, decidual cytosol extract, and serum. The assay is reproducible (intra-assay variation 4.3–8.2% and interassay variation 9.7–11.1%) and fast (< 5 hours). A crossreactivity of less than 0.01% for all other proteins tested reflects high specificity. Using this method the mean serum IGF-BP/PP12 concentration in healthy women was 5.2 μg/l. During pregnancy, the mean IGF-BP/PP12 at 7–11 weeks was 43.3 μg/l, and at 36–40 weeks 121 μg/l. After early pregnancy termination the serum IGF-BP/PP12 decreased rapidly reaching a mean level of 8 pg/l within 4 days.Keywords
This publication has 20 references indexed in Scilit:
- Dose-Response Characteristics for Suppression of Low Molecular Weight Plasma Insulin-Like Growth Factor-Binding Protein by Insulin*Journal of Clinical Endocrinology & Metabolism, 1989
- Primary structure of human insulin‐like growth factor‐binding protein/placental protein 12 and tissue‐specific expression of its mRNAFEBS Letters, 1988
- The biological and structural characterization of specific serum binding proteins for the insulin-like growth factorsJournal of Endocrinology, 1988
- Monoclonal antibodies to the 27–34K insulin-like growth factor binding proteinBiochemical and Biophysical Research Communications, 1988
- Soluble 34K Binding Protein Inhibits the Binding of Insulin-like Growth Factor I to Its Cell Receptors in Human Secretory Phase Endometrium: Evidence for Autocrine/Paracrine Regulation of Growth Factor Action*Journal of Clinical Endocrinology & Metabolism, 1988
- Placental Protein 12 Is a Decidual Protein that Binds Somatomedin and Has an Identical N-Terminal Amino Acid Sequence with Sdmatomedin-Binding Protein from Human Amhiotic Fluid*Endocrinology, 1986
- Radioimmunoassay of placental protein 12: Levels in amniotic fluid, cord blood, and serum of healthy adults, pregnant women, and patients with trophoblastic diseaseAmerican Journal of Obstetrics and Gynecology, 1982
- A simple two-step procedure for the simultaneous isolation of corticosteroid binding globulin and sex hormone binding globulin from human serum by chromatography on cortisol-sepharose and phenyl-sepharoseThe Journal of Steroid Biochemistry and Molecular Biology, 1981
- Isolierung und Charakterisierung eines neuen plazentaspezifischen Proteins (PP12)Archiv für Gynäkologie, 1980
- THE PREPARATION OF 131I-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITYBiochemical Journal, 1963