In vitro cultivation of Choristoneura fumiferana (Clemens) (Lepidoptera:Tortricidae) tissues

Publisher Website
Google Scholar
Abstract
Embryonic tissue fragments of the spruce budworm, Choristoneura fumiferana (Clemens), survived in vitro for 6–7 weeks. Three- to five-day-old eggs, maintained at 22 °C, were used to obtain embryos which were cut into small pieces and maintained in drop cultures using Grace's medium. Explants contracted rhythmically for 3–4 weeks and stayed in good condition for a further 2–3 weeks. Explanted tissues produced three types of growth: hollow vesicles, continuous cell sheets, and free cells. The size of vesicles in some cases was 4–5 times that of the explant. The free cells increased in number considerably and formed good monolayers. The cultures deteriorated after 6–7 weeks. Ovarian tissue cultures of late sixth-instar larvae of this insect did not produce any growth, and deteriorated in 3–4 days.

This publication has 0 references indexed in Scilit: