Abstract
Muscle cells that contract spontaneously and rhythmically can be obtained from adult mouse myocardium. Contractions are observed immediately after homogenization in a solution that is ionically similar to intracellular fluid. Contraction frequency varies directly with temperature and decreases as a function of time after homogenization. At 16°C rhythmic relaxation and contraction occur for about 20 minutes. Contractions are dependent on the presence of adenosine triphosphate in the homogenization medium.
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