Immune Interferon Production by TH69, a Lyophilized Preparation ofStreptococcus faecalis, in Murine Spleen Cell Cultures

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Abstract
In mouse spleen cell cultures, TH69, a live Streptococcus faecalis (ATCC 31663) preparation, at a concentration of 20 μg/ml induced immune interferon (IFNγ) with molecular weight ranging from 20,000 to 40,000 daltons together with a small amount of IFNα/β. By using nonsensitized mouse spleen cells, the fact that both T‐cells and macrophages are required for this IFN production was established. When these spleen cells were obtained from mice sensitized 12 days earlier with 4 mg of TH69, twice as much IFN was produced than in cells obtained from nonsensitized mice. This increase was explained by the presence of both sensitized macrophages and T‐cells in a reconstitution experiment.