Abstract
The absorption of manganese by beetroot tissue is shown to be partially inhibited by the presence of carbon dioxide. Preliminary treatment of well-washed tissue slices with carbon dioxide also produces, after placing them in manganese solutions, an inhibition of absorption for a time similar to that shown by tissue slices when first excised from stored roots. It is suggested that an inhibitory substance is formed in bulky storage roots and tubers by dark fixation of accumulated respiratory carbon dixoide. On cutting thin slices from such organs, the removal of inhibitor may take place by diffusion into water during washing, by reversal of the fixation reaction when the partial pressure of carbon dioxide is reduced, and possibly also by oxidation to form a substance capable of stimulating manganese absorption when sufficiently high oxygen concentrations are available.

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