The induction of delayed type hypersensitivity to dinitrochlorobenzene in the chicken

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Abstract
Delayed hypersensitivity to DNCB in chickens is a dose‐dependent reaction. It could be induced by a single painting of the skin with 1 mg of DNCB/bird without adjuvant and could be elicited at a challenge site with 50 μg DNCB. The sensitisation lasted for several weeks, and was mediated by sensitised lymphoid cells which required the presence of autologous cells that adhered to plastic for the reaction to be transferred to other allogeneic birds. Doses above 1 mg/bird induced some form of suppression. The migration inhibition test could be used as an in vitro method to measure the DTH response and correlated with the DTH skin response. The histological appearance of skin lesions was characterised by the infiltration of a large number of granulocytes especially heterophils and some eosinophils, as well as large numbers of monocytes.