A rapid inexpensive method for the isolation of restrictable mitochondrial DNA from various plant sources
- 1 December 1984
- journal article
- Published by Springer Nature in Plant Cell Reports
- Vol. 3 (6) , 237-239
- https://doi.org/10.1007/bf00269301
Abstract
A simplified method for the isolation of mitochondrial DNA (mtDNA) of several plant species from either coleoptile or tissue cultured cells is described. The procedure does not require gradient ultracentrifugation or organic solvent extractions (such as phenol, chloroform, ether, etc.). Protoplast isolation is not required for the release of organelles from cell suspension cultured cells. The entire procedure can be performed in a single day and employs differential low speed centrifugations for isolation of mitochondria and differential precipitations for the recovery of restrictable DNA.Keywords
This publication has 14 references indexed in Scilit:
- Molecular analysis of genomic stability of mitochondrial DNA in tissue cultured cells of maizeTheoretical and Applied Genetics, 1984
- A heteroplasmic state induced by protoplast fusion is a necessary condition for detecting rearrangements in Nicotiana mitochondrial DNATheoretical and Applied Genetics, 1983
- Novel composition of mitochondrial genomes in Petunia somatic hybrids derived from cytoplasmic male sterile and fertile plantsMolecular Genetics and Genomics, 1983
- Callus formation from protoplasts of a maize cell cultureTheoretical and Applied Genetics, 1981
- The mitochondrial genome is large and variable in a family of plants (Cucurbitaceae)Cell, 1981
- The effect of spermidine on endonuclease inhibition by agarose contaminantsAnalytical Biochemistry, 1981
- Characterization of 3H-labeled supercoiled mitochondrial DNA from tobacco suspension culture cellsMolecular Genetics and Genomics, 1980
- Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusionNature, 1979
- Characterization of Cytoplasmic Diversity in Soybeans by Restriction Endonuclease Analysis1Crop Science, 1978
- Plant protoplast fusion and growth of intergeneric hybrid cellsPlanta, 1974