MAPPING OF A HUMAN GENETIC REGULATOR ELEMENT BY SOMATIC CELL GENETIC ANALYSIS
- 1 August 1970
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 66 (4) , 1220-1227
- https://doi.org/10.1073/pnas.66.4.1220
Abstract
The ES-2 esterase is a kidney-associated esterase and is expressed in a cloned mouse renal adenocarcinoma cell line. Somatic cell hybridization of the mouse renal adenocarcinoma with either mouse or human fibroblasts leads to the marked reduction of ES-2 esterase titer. The extinction of ES-2 esterase activity in mousexhuman hybrids is reversible. Extinction correlates with the presence of the human C(10) chromosome in the somatic cell hybrids; whereas reexpression of the ES-2 esterase is observed in hybrids which have lost C(10). Thus, the proposed regulator element involved is probably structurally linked to C(10); however, linkage to a member of the D or G groups cannot be completely excluded.Keywords
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