IDENTIFICATION OF 3β,21-DIHYDROXY-5-PREGNENE-20-ONE DISULFATE IN HUMAN URINE

Open Access
Publisher Website
Google Scholar
Abstract
In pregnancy urines and those of normal subjects who had received zinc-ACTH we isolated a polar steroid ester-sulfate. This conjugate had the same electrophoretic and chromatographic behavior as the most polar compound obtained by the action of pyridine-sulfur trioxide on 21-hydroxypregnenolone. The quantity of 21-hydroxypreg-nenolone found in the urines after zinc-ACTH was 70 [mu]g/L and 25 [mu]g/L in pregnancy. After administration of 280 mg of 21-hydroxypregnenolone 21-monoacetate to a normal subject we isolated in urine 3 mg of 21-hydroxypregnenolone disulfate identical to the isolated ester-sulfate from the urine after zinc-ACTH; 21-hydroxypregnenolone was found in neither the free nor the glucuronide fraction.