A New Method for the Local Irritation Test. I. Tissue Regeneration Test for Intramuscular Acetic Acid Injection

Open Access
Publisher Website
Google Scholar
Abstract
The healing process of local lesions after the intramuscular injection of acetic acid into the muscularis vastus lateralis in rabbits was examined in order to establish a new method for determining local irritation due to intramuscular injection of drugs. The healing period of a lesion apparently depends on its volume and the volume closely correlated with the volume index (V.I.), i.e., the product of the major and minor axes of the largest cross section and the length of the lesion. Equations establishing the relationship between the V.I. and healing period of injection-produced lesions were worked out, on the basis of which it was possible to calculate the theoretical healing period of lesions with considerable accuracy by using the V.I. values 2 and/or 7 days after the injection. There was a marked loss of muscle weight 15 days or more after the injection of a highly concentrated solution, whereas the atrophy-time curves flattened with the healing of lesions. Histopathologically, marked fibrosis and fatty infiltrations were observed within and in the surrounding lesions in markedly atrophied muscles.

This publication has 0 references indexed in Scilit: