Preliminary Partial Purification of Hen Liver Microsomal Stearoyl‐CoA Desaturase

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Abstract
Hen liver microsomal stearoyl‐CoA desaturase has been prepared in “soluble” form, either by extraction of freeze‐dried microsomal fractions with high ionic strength phosphate buffer or by treatment with non‐ionic detergents. On a discontinuous density gradient, desaturase activity was recovered in the lightest fraction which is rich in lipids. On agarose gel, the desaturase activity was excluded in the void volume and therefore had a particle weight greater than 4 × 106. Removal of some of the lipids of the microsomal fraction led to inability to solubilize the desaturase, but resulted in enhancement of enzymic activity. Complete removal of lipids was not possible with acetone‐water mixtures and preparations from which the maximum amount of lipid had been removed contained one quarter to one half of the original enzymic activity. This residual activity could not be restored to the original value by addition of micellar dispersions of lipids.

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