Specific and sensitive plate assay for bacterial lipases
- 1 January 1987
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 53 (1) , 211-213
- https://doi.org/10.1128/aem.53.1.211-213.1987
Abstract
A plate assay to detect bacterial lipase (EC 3.1.1.3) in a medium containing trioleoylglycerol and the fluorescent dye rhodamine B is presented. Substrate hydrolysis causes the formation of orange fluorescent halos around bacterial colonies visible upon UV irradiation. The logarithm of lipase activity from cell-free culture supernatants is linearly correlated with the diameter of halos, thereby allowing quantitation of lipase activities ranging from 1 to 30 nkat.This publication has 11 references indexed in Scilit:
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