INSULIN-RELEASING ACTIVITY AND SUCCESSFUL TRANSPLANTATION OF PANCREATIC-ISLETS PRESERVED BY TISSUE-CULTURE

  • 1 January 1978
    • journal article
    • research article
    • Vol. 83  (2) , 188-193
Abstract
Using organ culture, the insulin-releasing activity at the time of administration of glucose and various digestive tract hormones was studied for the purpose of clarifying the function of preserved pancreatic islets. Pancreatic islets preserved for 3-5 days were transplanted into the portal vein of rats with streptozotocin-induced diabetes. The islets of Langerhans responded well to glucose up to the 7th day of preservation and showed patterns similar to those of fresh pancreatic islets with respect to both the dose response and the time response. Preserved pancreatic islets of the pancreas had insulin-releasing activity almost equal to that of fresh pancreatic islets upon stimulation with glucagon, tolbutamide and various digestive tract hormones. Rats with streptozotocin-induced diabetes showed a marked improvement in blood glucose and urine glucose following transplantation of preserved pancreatic islets into the portal vein, and this effectiveness persisted for 6-8 wk.

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