Physiological Analysis of Flower Formation in Wedgwood Iris

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Abstract
A series of experiments is described in which buds and scales of iris bulbs were implanted in nutrient media and subjected to varying temperature treatments, in an attempt to analyse the effect of different endogenous growth factors and of gibberellic acid on flower formation. In isolated scales incubated at 13° C for one to three weeks much of a heat-stable flower-inducing compound and a small quantity of a second growth factor is produced. At 25.5° C no such substances are formed. It is shown that at different times during a prolonged lowtemperature treatment of iris bulbs, different growth factors are formed which activate successive stages in the differentiation of the flower primordium. At 25.5° C the production and activity of the factor responsible for the transition to the reproductive stage are inhibited. Probably not inhibited at this temperature are differentiation processes in the reproductive primordium controlled by other factors with gibberellin-like characters.

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