Fluorometric assay of vasopressin and oxytocin: a general approach to the assay of peptides in tissues.
- 1 April 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (4) , 1314-1318
- https://doi.org/10.1073/pnas.73.4.1314
Abstract
A fluorometric method for the quantitative assay of vasopressin and oxytocin in individual rat pituitaries was developed. Acid extracts of pituitaries are freed of amino acids and polyamines by passage over a copper-Sephadex column, and the peptide fraction is then labeled by reaction with fluorescamine. The resulting peptide fluorophors are separated by chromatography on a reverse-phase bonded column. Specificity of the procedure was ascertained by several criteria, including bioassay and amino-acid analysis of the eluted peptide fluorophors. The procedure serves as a model system for the assay of tissue peptides in the picomole range.This publication has 9 references indexed in Scilit:
- Automatic monitoring of primary amines in preparative column effluents with fluorescamineAnalytical Biochemistry, 1975
- Removal of a fluoram-positive impurity from hydrochloric acidAnalytical Biochemistry, 1974
- Amino acid analysis with fluorescamine of stained protein bands from polyacrylamide gelsAnalytical Biochemistry, 1974
- A simplified protein assay with fluorescamine in samples containing interfering materialAnalytical Biochemistry, 1974
- Fluorescent labeling of proteins. A new methodologyBiochemical and Biophysical Research Communications, 1973
- Amino acid analysis with fluorescamine at the picomole levelArchives of Biochemistry and Biophysics, 1973
- Fluorescamine: A Reagent for Assay of Amino Acids, Peptides, Proteins, and Primary Amines in the Picomole RangeScience, 1972
- Supraoptic Neurosecretory Neurons of the Guinea Pig in Organ Culture. Biosynthesis of Vasopressin and NeurophysinProceedings of the National Academy of Sciences, 1971
- Separation of amino acids, as copper chelates, from amino acid, protein, and peptide mixturesAnalytical Biochemistry, 1965