A Simplified Method for Peroxyoxalate Chemiluminescence Detection of Fluorescers Based on Solid State Reagent Addition

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Abstract
A peroxyoxalate chemiluminescence detection system which needs no reagent-pumps is described. It is based on solid state addition of bis-(2,4,6,trichlorophenyl)oxalate (TCPO) from a solid state reagent bed. The solid reagent is packed in a column and situated parallel to the analytical column in order to minimize band broadening. If chromatographic separation can be achieved at conditions matching those of the chemiluminescence reaction (>80% acetonitrile) a split-flow system utilizing only the mobile phase pump can be employed. For mobile phase compositions with at least 50% acetonitrile a system with only one reagent pump is developed. The performance of both post-column detection systems are compared to that of a conventional three pump system as described by Sigvardson et al.[5]