Deoxyribonucleic acids (DNA) and related compounds. XIII. Synthesis of DNA duplexes containing a ribosome binding site.
- 1 January 1985
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 33 (8) , 3153-3159
- https://doi.org/10.1248/cpb.33.3153
Abstract
To investigate the efficiency of expression of synthetic genes, deoxyribonucleic acid (DNA) duplexes (33-37 base pairs) containing the ribosome binding site (S.D. [Shine-Dalgarno] sequence) for trpL and appropriate termini were synthesized. This involved chemical synthesis of 8 oligonucleotides with chain lengths of 16-19 by the solid-phase phosphotriester method followed by enzymatic joining with DNA ligase. The duplexes were designed to replace a restriction fragment from a plasmid containing the trp promoter and a synthetic human growth hormone gene. The S.D. sequence in these duplexes was located upstream from a Cla I site to make a distance of 9, 11 or 13 bases from the initiation signal (ATG).This publication has 6 references indexed in Scilit:
- Synthesis of a gene for human growth hormone and its expression in Escherichia coli.Proceedings of the National Academy of Sciences, 1984
- Production in Escherichia coli of biologically active secretin, a gastrointestinal hormone.Proceedings of the National Academy of Sciences, 1982
- Total synthesis of a human leukocyte interferon geneNature, 1981
- Direct expression in Escherichia coli of a DNA sequence coding for human growth hormoneNature, 1979
- Expression in Escherichia coli of a Chemically Synthesized Gene for the Hormone SomatostatinScience, 1977
- Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli.Proceedings of the National Academy of Sciences, 1965