Efficient Gene Transfer and Expression of Biologically Active Glial Cell Line‐Derived Neurotrophic Factor in Rat Motoneurons Transduced with Lentiviral Vectors
- 1 May 2000
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 74 (5) , 1820-1828
- https://doi.org/10.1046/j.1471-4159.2000.0741820.x
Abstract
Several studies have shown the ability of human immunodeficiency virus type 1 (HIV1)-based lentiviral vectors to infect nondividing brain and retinal neurons with high efficiency and long-term expression of the transduced gene. We show that purified embryonic motoneurons can be efficiently (>95%) transduced in culture using an HIV1-based lentiviral vector encoding LacZ. Expression of β-galactosidase was observed for at least 9 days in these conditions. Furthermore, motoneurons transduced with a lentiviral vector expressing glial cell line-derived neurotrophic factor survived in the absence of additional trophic support, showing that the overexpressed protein was biologically active. Our results demonstrate the potential of lentiviral vectors in studying the biological effects of proteins expressed in motoneurons and in the development of future gene therapy for motoneuron diseases.Keywords
This publication has 61 references indexed in Scilit:
- Self-Inactivating Lentiviral Vectors with Enhanced Transgene Expression as Potential Gene Transfer System in Parkinson's DiseaseHuman Gene Therapy, 2000
- Continuous delivery of human and mouse erythropoietin in mice by genetically engineered polymer encapsulated myoblastsGene Therapy, 1998
- Gene therapy—the gutless approach pays offTrends in Genetics, 1998
- In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral VectorScience, 1996
- Loss of Expression of a Retrovirus-Transduced Gene in Human KeratinocytesJournal of Investigative Dermatology, 1996
- Sustained expression of functional nerve growth factor in primary septo-hippocampal cell cultures by liposome-mediated gene transferNeuroscience Letters, 1994
- Nerve growth factor protects against herpes simplex virus type 1 neurotoxicity in the rat striatumNeuroReport, 1994
- High-Level Human Adenosine Deaminase Expression in Dog Skin Fibroblasts Is Not Sustained Following TransplantationHuman Gene Therapy, 1993
- Long-term expression of a retrovirally introduced ?-galactosidase gene in rodent cells implanted in vivo using biodegradable polymer meshesSomatic Cell and Molecular Genetics, 1992
- HIV-1 entry into quiescent primary lymphocytes: Molecular analysis reveals a labile, latent viral structureCell, 1990