The use of nuclease P1in sequence analysis of end group labeled RNA
- 1 January 1977
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 4 (12) , 4091-4108
- https://doi.org/10.1093/nar/4.12.4091
Abstract
A method is described for the direct sequence analysis of 20-25 nucleotides from the termini of 5'- or 3'-end-group [32P] labeled RNA. The method involves partial endonucleolytic digestion of the labeled RNA with nuclease P1 (from Penicillium citrinum) followed by separation of the partial digestion products by two-dimensional homochromatography, the nucleotide sequence being determined by mobility shift analysis. This procedure has been applied to the sequence analysis of the terminal regions of tRNAs and of high molecular weight RNA, such as messenger RNA or viral RNA. A further application involves its use in conjunction with snake venom phosphodiesterase to determine the sequence of 5'-end group labeled oligonucleotides, containing modified bases, derived from T1 or pancreatic RNase digestion of tRNA.Keywords
This publication has 22 references indexed in Scilit:
- TUMORIGENIC ACTION OF STREPTOZOTOCIN ON PANCREAS AND KIDNEY IN MALE WISTAR RATS1978
- Studies on the Sequence of the 3′‐Terminal Region of Turnip‐Yellow‐Mosaic‐Virus RNAEuropean Journal of Biochemistry, 1977
- Nucleotide sequences at the 5$prime; termini of rabbit $alpha; and $beta; globin mRNACell, 1976
- Capping of eucaryotic mRNAsCell, 1976
- The first nucleotide sequence of an organelle transfer RNA: Chloroplastic tRNAPheCell, 1976
- Nucleotide sequence of salmon testes and salmon liver cytoplasmic initiator tRNACell, 1975
- Nucleotide sequence of human placenta cytoplasmic initiator tRNACell, 1975
- The Primary Structure of DNAProgress in Nucleic Acid Research and Molecular Biology, 1974
- Polynucleotides. CXXIII. Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymeraseBiochemistry, 1973
- Resolution of Multiple Ribonucleic Acid Species by Polyacrylamide Gel Electrophoresis*Biochemistry, 1967