A seven-base-pair deletion in an intron of the albumin gene of analbuminemic rats.

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Abstract
Analbuminemic rats, which genetically lack serum albumin, have a mutation affecting albumin mRNA processing. Serum albumin genes were cloned from analbuminemic and normal parental Sprague-Dawley rats. Structural analyses of the 2 albumin genes showed that the gene from analbuminemic rats had a 7 basepair deletion in an intron. The deletion extended from base 5 to base 11 from the 5'' end of intron HI of the albumin gene. This deletion converted the sequence G-T-A-G-G-T, which is normally located at the 5'' end of intron HI, to G-T-A-G-C-G. RNA blot hybridization of analbuminemic and normal rat liver nuclear RNA using a DNA fragment containing the intron HI as a probe showed that this intron sequence persisted in albumin mRNA precursors of analbuminemic rats.