Promotion of porphyrin cytotoxicity by ultrasound (Invited Paper)

1 June 1992

proceedings article
Published by SPIE-Intl Soc Optical Eng

p. 82-90
https://doi.org/10.1117/12.60930

Abstract

We examined the effect of ultrasound on the murine leukemia L1210 cell line in culture, in the presence of different photosensitizing agents: mesoporphyrin and tetraphenylporphines bearing 1 - 4 sulfonate groups. These agents could enhance ultrasound-induced cell damage when added to the incubation medium; sensitizers present only in the intracellular space were inactive in this regard. All of the porphine sulfonates were equally effective sensitizers to ultrasound but the product with 2 adjacent-SO₃H groups was the most effective photodynamic sensitizer. Ultrasound-induced cytotoxicity was associated with outer membrane damage and could be detected by measuring hydrophobic- or charge-associated whole-cell partitioning or by measurement of inhibition of active transport of a non-metabolized amino acid.

Keywords

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