Phosphatase toward MAP kinase is regulated by osmolarity in Madin‐Darby canine kidney (MDCK) cells
Open Access
- 9 October 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 373 (2) , 123-126
- https://doi.org/10.1016/0014-5793(95)01028-d
Abstract
We have reported that MAP kinase and its activator were activated by increase in extracellular osmolarity in Madin‐Darby canine kidney (MDCK) cells [J. Clin. Invest. 93 (1994) 2387–2392]. The activation of MAP kinase quickly disappeared when cells in hypertonicity were shifted to isotonicity. Present study was planned to elucidate the mechanism for the inactivation of MAP kinase when osmolarity decreased. Combination of two different phosphatase inhibitors, 10−6 M okadaic acid and 0.2 mM sodium orthovanadate, blocked the inactivation of MAP kinase after the decrease in osmolarity. We also demonstrated that phosphatase toward MAP kinase was activated in response to the decrease in osmolarity. These results suggest that MAP kinase is inactivated by phosphatase that is activated when osmolarity decreased.Keywords
This publication has 20 references indexed in Scilit:
- MAPKs: new JNK expands the groupPublished by Elsevier ,2002
- Activation of Fes Tyrosine Kinase by gp130, an Interleukin-6 Family Cytokine Signal Transducer, and Their AssociationPublished by Elsevier ,1995
- Mitogen-activated Protein (MAP) Kinase Is Regulated by the MAP Kinase Phosphatase (MKP-1) in Vascular Smooth Muscle CellsPublished by Elsevier ,1995
- The Purification and Characterization of a Human Dual-specific Protein Tyrosine PhosphataseJournal of Biological Chemistry, 1995
- A MAP Kinase Targeted by Endotoxin and Hyperosmolarity in Mammalian CellsScience, 1994
- Mitogen-activated protein kinase and its activator are regulated by hypertonic stress in Madin-Darby canine kidney cells.Journal of Clinical Investigation, 1994
- A two-component system that regulates an osmosensing MAP kinase cascade in yeastNature, 1994
- The stress-activated protein kinase subfamily of c-Jun kinasesNature, 1994
- Na,K-ATPase expression and cell volume during hypertonic stress in human renal cellsKidney International, 1993
- An Osmosensing Signal Transduction Pathway in YeastScience, 1993