THE DISTRIBUTION OF CHROMATIN IN THE INTERPHASE NUCLEUS OFZEBRINA PENDULA

Abstract
An investigation of nuclear organization in interphase root tip cells of Zebrina pendula Schnizl. showed that: (1) 40% of the BSG (Barium hydroxide/saline/Giemsa) treated nuclei had nonrandomly distributed chromocenters and (2) BrdU-FPG (5′-bromodeoxyuridine-fluorescence plus Giemsa) treated nuclei showed discrete staining patterns when exposed to BrdU for time intervals of two or more cell cycles. These data were interpreted as further evidence for an ordered spatial arrangement of chromosomal regions in the interphase nucleus.