Folding of barstar C40A/C82A/P27A and catalysis of the peptidyl‐prolylcis/transisomerization by human cytosolic cyclophilin (Cyp18)
- 1 January 1999
- journal article
- Published by Wiley in Protein Science
- Vol. 8 (7) , 1505-1514
- https://doi.org/10.1110/ps.8.7.1505
Abstract
Refolding of b*C40A/C82A/P27A is comprised of several kinetically detectable folding phases. The slowest phase in refolding originates fromtrans → cisisomerization of the Tyr47–Pro48 peptide bond being incisconformation in the native state. This refolding phase can be accelerated by the peptidyl‐prolylcis/transisomerase human cytosolic cyclophilin (Cyp18) with akcat/KMof 254, 000 M–1s–1. The fast refolding phase is not influenced by the enzyme.Keywords
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