Isolation of the intermediate filament protein vimentin by chromatofocusing
- 28 January 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 180 (2) , 181-184
- https://doi.org/10.1016/0014-5793(85)81067-x
Abstract
A novel, simple and relatively rapid method is described for the isolation of the intermediate-sized filament protein vimentin from eye lens tissue. Chromatofocusing is applied as the sole purification step. The apparent isoelectric point of the protein in 6 M urea and at 22°C is 4.9. Electrophoretic mobility on one- and two-dimensional polyacrylamide gels, solubility in 6 M urea and amino acid composition were used for identificatioKeywords
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