Metabolic responses of Sertoli cells in culture to various concentrations of follicle stimulating hormone and cholera toxin

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Abstract
The concentration of cholera toxin required for half-maximal stimulation of c[cyclic]AMP production by Sertoli cell enriched cultures (4.48 .times. 10-2 .mu.g/ml) is greater than that required for half-maximal stimulation of 17.beta.-estradiol synthesis from testosterone (2.34 .times. 10-4 .mu.g/ml), [3H]thymidine incorporation into DNA (1.48 .times. 10-5 .mu.g/ml), or androgen binding protein production (2.43 .times. 10-6 .mu.g/ml). The same relative dose response hierarchy was obtained with respect to stimulation of Sertoli cells with ovine follicle-stimulating hormone (FSH) preparations. Again, highest concentrations were required to elicit maximal cAMP production. The data are discussed in relation to an apparent paradox: if cAMP is the mediating second messenger following stimulation by FSH or cholera toxin, why should highest concentrations of these agents be required to elicit 50% of maximal cAMP levels?

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