Establishment and Characterization of Acute B-Cell Lymphocytic Leukemia Cell Line Showing (8; 14) and (14; 18) Chromosome Translocation

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Abstract
Cell line KHM-2B expressing two oncogene products, c-myc and bcl-2, was established from a patient with actute lymphocytic leukemia with an 8; 14 and 14; 18 chromosome translocation. Surface marker studies of the cell line showed that the cells were positive for HLA-DR, CALLA (CD10), Bl (CD20) and B4 (CD19), but negative for Tl 1 (CD2). The fresh cells from peripheral blood of the patient had no surface immunoglobulins, whereas KHM-2B cells were positive for μ·λ type surface immunoglobulin. A cytogenetic analysis of the cell line revealed two translocations, t (8;14) (q24;q32) and t( 14; 18)(q32;q21). Rearrangement of the c-myc and bcl-2 genes was detected by Southern blot analysis of the KHM-2B DNA. Northern blot analysis revealed production of c-myc and bcl-2 mRNAs. These results indicated that two oncogenes were activated by two translocations to immunoglobulin genes.

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