ERYTHROCRUORIN OF MARPHYSA SANGUINEA

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Abstract
The polychaete worm Marphysa sanguinea has a circulating erythrocruorin of mol. wt. about 2-4 x 106 (S0 20.w 58-2 s, D20,w 2.06 x 10-7 cm.2/sec). This is the predominant form existing at pH 6-8 and (non-protein) I0.10-0[center dot]21, and also at approx. pH 6-7 and I0[center dot]15-3[center dot]00. The pigment contains 2.24% of protohaem. The 58 s protein has an electrophoretic mobility of 8-08x10-5 cm.2/v/sec. at pH 8[center dot]12, 10[center dot]21 and 0[degree]. The isoelectric point of suspendedparticles is 4[center dot]63 at I0[center dot]16 and 21[center dot]5[degree]. At very low ionic strength and pH 6[center dot]7 (unbuffered) the 58spigment associates reversibly to 97 s and 150 s forms, which are probably dimer and tetramer species. At pH 10[center dot]0 and I0[center dot]025, it dissociates irreversibly to give a small amount of 2-4 s non-haem-containing protein and much 9 s haem-enrichedprotein. These and the 58 s pigment may corres-pondto structuresfound in Levin''s (1963) electron-microscope studies of other erythrocruorlns. Absorption spectra of the 58 s oxygenated erythrocruorin and the deoxygenated and carbon monoxide derivatives have been obtained.

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