Morphology of Mycoplasma laidlawii Type A I. Comparison of Electron Microscopic Counts with Colony-Forming Units
- 1 December 1965
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 90 (6) , 1764-+
- https://doi.org/10.1128/jb.90.6.1764-1767.1965
Abstract
Cells of M. laidawli A grown in dialyzing flask cultures were counted with the electron microscope by use of a spray technique which deposited mixtures of polystyrene latex of known concentration and M. laidlawii in discrete droplet patterns on specimen films. Glutaraldehyde and formaldehyde were effective in preserving gross morphology of cells in spray preparations. The standard deviation of the mean ratio of latex-M. laidlawii was 5.5% when 2,000 total particles were counted in 18 droplet patterns. Microscopic counts resembled counts of colony-forming units (CFU) at various culture ages when cells larger than 0.25 [mu] were enumerated. If small bodies ranging from 0.1 to 0.25 [mu] in diameter were included in microscopic counts of cultures older than 70 hr., these counts exceeded the numbers of CFU by 4 to 10 times.This publication has 4 references indexed in Scilit:
- Use of Dialyzing Culture Technique for High Yield of MycoplasmaJournal of Bacteriology, 1965
- The Smallest Living CellsScientific American, 1962
- Determination of Particle Size by FiltrationPublished by Elsevier ,1961
- The Use of Spraying Methods and of Volatile Suspending Media in the Preparation of Specimens for Electron MicroscopyJournal of Applied Physics, 1950