Pressure Dependence of l-Leucine-p-nitroanilide Hydrolysis by Leucine Aminopeptidase
- 1 April 1985
- journal article
- Published by Oxford University Press (OUP) in Bulletin of the Chemical Society of Japan
- Vol. 58 (4) , 1349-1350
- https://doi.org/10.1246/bcsj.58.1349
Abstract
The effect of pressure on the hydrolysis of l-leucine-p-nitroanilide catalyzed by leucine aminopeptidase from pig kidney was investigated. The activation volumes for kcat process were −8 cm3/mol and −9 cm3/mol for the native enzyme and the modulated one by Mg2+, respectively. The volume changes for the Km process were \simeq0 cm3/mol for both enzymes.Keywords
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