Exposure to power frequency magnetic fields and X‐Rays induces GAP‐43 gene expression in human glioma MO54 cells

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Abstract
We investigated the distribution and expression of growth associated protein‐43 (GAP‐43) in human glioma cells (MO54) after exposure to a magnetic field (60 Hz, 5 mT), with or without initial X‐ionizing radiation (2 Gy), by using immunocytochemistry and the reverse transcription polymerase chain reaction (RT‐PCR). GAP‐43 was present in the cytoplasm, accumulating in the perinuclear area. An increase in GAP‐43 expression was observed with a peak at 10 h at the mRNA level and at 12 h at the protein level, after exposure to the magnetic field. The increased level of GAP‐43 protein returned to a normal level within 24 h of exposure to a 5 mT magnetic field. The kinetic pattern of GAP‐43 expression induced by X‐ionizing radiation was very similar to that induced by the magnetic field. These results suggest that the stimulation of GAP‐43 expression could occur by a similar mechanism following exposure to X‐rays or magnetic fields. We have provided the first evidence that exposure to a 5 mT magnetic field can induce GAP‐43 gene expression in human glioma MO54 cells. Bioelectromagnetics 23:586–591, 2002.

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