Oligonucleotide fingerprints of RNA species obtained from rhabdoviruses belonging to the vesicular stomatitis virus subgroup

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Abstract
The relationships among the genomes of various rhabdoviruses belonging to the vesicular stomatitis virus [VSV] subgroup were analyzed by an oligonucleotide fingerprinting technique. Of 10 vesicular stomatitis viruses, Indiana serotype (VSV Indiana), obtained from various sources, either no, few or many differences were observed in the oligonucleotide fingerprints of the 42S RNA species extracted from standard B virions. Analyses of the oligonucleotides obtained from RNA extracted from 3 separate preparations of VSV Indiana defective T particles showed that their RNA contain fewer oligonucleotides than the corresponding B particle RNA species. The fingerprints of RNA obtained from 5 VSV New Jersey serotype viruses were easily distinguished from those of the VSV Indiana isolates. Three of the VSV New Jersey RNA fingerprints were similar to each other but quite different from those of the other 2 viruses. The RNA fingerprints of 2 Chandipura virus isolates (one obtained from India and one from Nigeria) were also unique, but the fingerprint of Cocal virus RNA was unlike that of the serologically related VSV Indiana.