A chicken leukocyte common antigen: biochemical characterization and ontogenetic study

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Abstract
A monoclonal antibody, designated CL‐1, was produced by immunizing mice with chicken peripheral blood lymphocytes (PBL) and selected in an indirect immuno‐fluorescence assay. CL‐1 characterizes a cell surface antigen expressed on all chicken hemopoietic cells except mature erythrocytes. Cells stained by CL‐1 were detected at early stages of embryonic development in the various hemopoietic compartments. CL‐1 reacts with two chicken PBL proteins of 200 kDa and 215 kDa which are probably the homologues of the T‐200 and B‐220 antigens previously described in the mouse. Moreover, CL‐1 does not react with quail hemopoietic tissues and can be useful in experiments using quail‐chick chimeras to study the ontogeny of the hemopoietic system.

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